somatic cell nuclear transfer; cell fusion; livestock iPSCs; genome editing
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  • Monash Institute of Medical Research

Research interests

Dr Liu's research interests focus on:

  1. Generation of integration-free human induced pluripotent stem cells (iPSCs) to develop safe, clinically relevant cells for cell therapy.
  2. Establishment of pluripotent embryo-derived cell lines from the NOD mouse (a non-permissive strain of mice), an animal model for the study of autoimmune disease such as Type 1 diabetes (T1D). Small molecule inhibitors of ERK1/2 and GSK3 signalling pathways, to isolate and maintain NOD ESCs with greater efficiency have been identified. Further research is aimed at investigating IDD candidate genes that have been linked with T1D.
  3. Exploring conditions to generate induced pluripotent stem cells (iPSCs) in livestock, which provide a unique source of cells from large animals that allow specific traits to be engineered for agricultural or pharmaceutical purposes, and study developmental biology and regenerative medicine.

Publications

  1. Liu J, Balehosur D, Murray B, Kelly JM, Sumer H, Verma PJ. Generation and characterization of reprogrammed sheep induced pluripotent stem cells. Theriogenology 2011; (in press).
  2. Liu J, Aston MP, Sumer H, O’Bryan MK, Brodnicki TC, Verma PJ. Generation of pluripotent stem cells from Non-Obese Diabetic (NOD) mouse tail-tip fibroblasts. Diabetes 2011; 60:1393-1398.
  3. Liu J, Verma PJ, Evans-Galea M, Delatycki M, Michalska A, Leung J, Crombie D, Sarsero J, Williamson R, Dottori M, Pébay A. Generation and function of induced pluripotent stem cell lines from Friedreich Ataxia patients. Stem Cell Review and Report 2011; 7:703-713.
  4. Liu J, Sumer H, Leung J, Upton K, Dottori M, Pébay A, Verma PJ. Late passage human fibroblasts induced to pluripotency are capable of directed neuronal differentiation. Cell Transplantation 2011; 20:193-203.