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  • Director and Group Leader, Australian Institute for Bioengineering and Nanotechnology

Research interests

Professor Gray's research is focused on engineering mammalian cells in order to improve their efficiency and utility in the production of complex proteins, increasingly being used as biopharmaceuticals. Professor Gray’s research is aimed at reducing some of the 'bottlenecks' present when mammalian cells are used to produce biopharmaceuticals, viz:

  1. Developing transient protein expression systems which will allow researchers to rapidly produce larger amounts of protein needed for initial characterisation and testing
  2. Developing high throughput approaches which allow the rapid selection of clones which stably express high levels of the desired biopharmaceutical
  3. Using modern 'omics' approaches to gain better understanding of cellular metabolism which will allow maximal protein expression by mammalian cell cultures.

The research approaches which have been used to gain a greater understanding of mammalian cell processes are now being applied to even more complex cells, viz the development of bioprocesses based on embryonic stem cells. With stem cells the challenge is to accurately define the physical and chemical environment which allows the controlled proliferation and subsequent differentiation of the cells, and then translate these conditions into processes which can be scaled up to produce the number of cells which will be required for clinical testing.

Publications

  1. Prowse AB, Doran MR, Cooper-White JJ, Chong F, Munro TP, Fitzpatrick J, Chung TL, Haylock DN, Gray PP, Wolvetang EJ. (2010). Long term culture of human embryonic stem cells on recombinant vitronectin in ascorbate free media. Biomaterials. Nov;31(32):8281-8.
  2. Doran MR, Frith JE, Prowse AB, Fitzpatrick J, Wolvetang EJ, Munro TP, Gray PP, Cooper-White JJ. (2010). Defined high protein content surfaces for stem cell culture. Biomaterials. 2010 Jul;31(19):5137-42.
  3. Prowse AB, Wilson J, Osborne GW, Gray PP, Wolvetang E. (2009) Multiplexed Staining of Live Human Embryonic Stem Cells for Flow Cytometric Analysis of Pluripotency Markers. Stem Cells Dev. Apr 27.
  4. Prowse AB, McQuade LR, Bryant KJ, Marcal H, Gray PP. (2007) Identification of potential pluripotency determinants for human embryonic stem cells following proteomic analysis of human and mouse fibroblast conditioned media. J Proteome Res. Sep;6(9):3796-807.
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